1. Abstract
整體要求簡練,言簡意賅,因為絕大部分 journal 對 Abstract 都有字數限制,一般150~250 words. 先簡單描述一下自己工作的前沿背景,幾句話,然後引出自己的實驗(“In this paper...”; “Herein...” or “Here, we...”),主要陳述實驗結果,可稍帶提一下所用到的重要方法的名稱,然後說明你的結果的意義 “These data suggest...”;最後總結拔高 “In a word,...” or “In summary,...”.
2. Introduction
描述跟你的實驗相關的領域研究現狀及存在的問題,自然的過度到你實驗的意義和重要性。“Although..., there are still... so far” “Here, for the first time, we have used...”。如果你前面已發過相關的文章,要插入引用一下 “We recently showed that...” 或 “Our previous studies have shown that.... These results verify that...” “Here...” “To explore this hypothesis, we generated...”. 寫好Introduction需要有大量的參考文獻做基礎,摘取短語、詞組、句子進行 reorganize。調節好前後句子的邏輯順序及銜接。
3. Materials and Methods
這部分可以從別的文章摘到現成的方法描述,加以改造,替換成自己的實驗條件,變成自己的表達方式,近幾年 journal 對抄襲句子的現象抓得特別嚴,多注意。太複雜的經典方法,可以簡單描述後加上參考文獻“Briefly, ... [ref]”;也可以直接引用參考文獻 “The...method was performed as described previously [ref]”.
4. Results
這一部分很重要。要按照一定的邏輯順序進行 organize,結果描述要準確、專業,一些重要的數據可以放在結果部分的文字描述中,如 IC50、EC50、抑制百分率、P 值等等。這部分不能像 Introduction 那樣寫太多的理論性的東西。每段的開頭或一些連接的地方可以簡單地用一些理論性的或總結性的句子。在陳述你的實驗操作和看到的結果和表面現象時用過去時態,陳述不以人的意志為轉移的規律、結論性的內容時用一般現在時。
一些常用的短語及句式有:
“In order to prove..., we used...”;
“We have set up...”;
“To demonstrate..., we further...”;
“Consistently/Consistent with...”;
“Compared with...”;
“Thus, at current time, we have evidence that...”;
“We next characterized...”;
“We found that...”;
“We have noticed that...”;
“It's known that...”;
“So we introduced... In our study...”;
“In contrast...”;
“These data suggest that...”;
“So we next explored...”;
“Notably, ...”;
“Importantly, ...”;
“Furthermore...”;
“Moreover...”;
“We have previously shown that...”;
“As shown in Fig. 1, ...”;
“Fig. 1 shows...”;
“Overall, ...”;
“Taken together, these results suggest...”;
“These data are consistent with the notion that...”;
“Next, we examined the effect of...”;
“We next set out to determine whether...”;
“Lastly, we examined...”;
“In order to establish...”.
5. Discussion
這一部分最重要,也最難寫,是大家最容易犯錯的地方。即使是對一些發了很多文章的老教授來說,這部分仍然是塊硬骨頭,尤其是 top paper,Discussion 非常重要,因為評閱你 paper 的人,都是世界頂級的大牛。
首先要注意,Discussion 不能過多地羅列和重複結果部分的內容,這是常犯的錯誤,因為感覺沒什麼可說的,就重複結果來充數。討論部分可以就你的實驗結果論述與你的結果密切相關的研究現狀存在的一系列問題,你的工作是解決的哪一部分問題。
討論要延伸出很多結果部分所體現不出來的信息,如你的工作的長期效應、潛在效應、與他人工作的比較,相同之處、不同之處、你的優勢、你的結果存在的問題、侷限性及其原因、將來可以改進的地方等等。也可以就你實驗中得到的跟預期不一致的 interesting 的結果簡單討論一下可能的原因。
對一些 top 的 paper 來說,你的 research 中的一些問題是很難躲得掉的,還不如主動提出討論,變被動為主動。對一般的文章,另當別論,一般大家都喜歡畫一個圓滿的圈。寫作風格上每個人不一樣,有溫文爾雅、按部就班的,也有如西部牛仔一樣豪放的,都沒問題。 討論開始,可以開門見山:“Here, we describe....” 或 “In this paper, we show that.... Importantly, ..., suggesting...”
下面是一些常用的短語和句式:
“Overall, our studies establish the...”;
“Although there are important discoveries revealed by these studies, there are also limitations. First, ...;Second, ...;Third, ...;Fourth, ...;Last, ...Overall, ...”;
“Furthermore, our results suggest that...”;
“Our results suggest a possibility of...”;
“One important future direction of ...is...”;
“To this end, we show that...”;
“However, none of these approaches to date holds the...”;
“Our studies serve as a proof-of-concept that...”;
“This could explain why...”;
“Alternatively, ... may be operative...”;
“Thus, future iterations of ... may in fact demonstrate even greater potency.”;
“On the other hand, the lack of...”;
“In summary, we have identified...”;
“Our results confirm that...”;
“These studies thus offer a new strategy to treat...”
6. Acknowledgements
這一部分主要感謝友情給你幫助的,如給過你質粒、細胞或其它材料的,技術服務及寫作指導的,最後寫基金號,寫多少都行。
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