比如審稿人提出：裂解液成分有問題
回覆：We are very sorry for the mistake. Lentivirus-transduced cells were washed twice with PBS, and suspended in a lysis buffer (2% mercaptoethanol, 20% glycerol, and 4% SDS, in 100 mM Tris-HCl buffer, pH 6.8). The information for the lysis buffer has been corrected in the materials and methods.
技巧3：補充實驗，耗時耗力，如果不補，只能argue
比如審稿人提出：補充正常皮膚細胞作對照
Tips：如果你真的不想補，或者正常細胞株，你也買不到，還是查一查正常細胞株的名字，告訴審稿人你確實是去努力過，只是事與願違啊！
回覆：Indeed, it will be more convincing if we get a comparative assessment on normal keratinocytes. However, the normal skin cell line TE 353.SK that we recently obtained could not be cultured stably under the condition of our lab at this time. We are also collecting human tissue samples from patients with primary melanoma and benign nevi. Based on our data, XXX expression will be analyzed in clinical samples by immunohistochemistry, and the correlation between XXX expression and the prognosis of melanoma will be determined in our subsequent investigations. Therefore, the referee’s concern is of importance for our further study, and we will show the results in our next paper for XXX.
比如審稿人提出：補充體內實驗
Tips：補充動物實驗，是審稿人最愛發的一招，其實一點不令人意外。既然躲不過，就要見招拆招，如果能補上，當然是給了漂亮的回馬槍；要是沒有條件補，或者實驗來不及，也要儘量說的委婉點，假裝你確實努力了，是審稿人給的時間太短了，我根本來不及啊！
回覆：We are appreciative of the reviewer’s suggestion. Indeed, it will be more profound if we get the relevant results in vivo. We have purchased 10 thymus-null BALB/c nude mice (female, age 4-6 weeks) from the Animal Center of Chinese Academy of Medical Sciences. Osteosarcoma xenografts were established in nude mice according to a previous report (Gao et al., 2009) （我們去買了若干只小鼠，按照文獻的方法操作）. Briefly, control lentivirus or XXX gene shRNA-expressing lentivirus infected XXX cells (1×105) were suspended in 0.1 ml PBS and then injected subcutaneously into the proximal tibia of each anesthetized nude mice (n=5 in each group). Unfortunately, the xenografts could not grow after inoculation for three weeks （滿懷欣喜的接種到動物身上，然而實驗這東西，哪能一遍就成呢，我們做了一遍，結果沒能成瘤，實在是悲劇了）. The limitation of technical condition may be the main reason. Also, we doubt that the cell viability could be insufficient to initiate osteosarcoma tumorigenesis in vivo due to the long-distance transport. Therefore, we seek for the editor’s tolerance and understanding. Many thanks for your kind help!
然而，補充實驗，還是逃不過的一劫，究竟是申請延期，再老老實實把實驗做完，還是想投機取巧，argue一下，就看自己的運氣了。一般情況，可以對照審稿人的意見綜合權衡。高分文章，最好還是該補的全補了，低分文章，如果你覺得有80%的把握，完全可以憑你的勁爆人品成功拿下審稿人。
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