Comment #5: 裂解液成分有問題
回覆：We are very sorry for the mistake. Lentivirus-transduced cells werewashed twice with PBS, and suspended in a lysis buffer (2% mercaptoethanol, 20%glycerol, and 4% SDS, in 100 mM Tris-HCl buffer, pH 6.8). The information forthe lysis buffer has been corrected in the materials and methods.
遇到補實驗，怎麼辦？
當然，在輕鬆搞定這些論文細節的修改後，同學們最最擔心的就是審稿人會認為實驗數據不夠分，不足以支撐整個論文，要求補實驗！比如：你丫怎麼就用了一個細胞系，這個問題的說明起碼要兩個細胞系，快去補！
相信此時各位小夥伴的內心是崩潰的，補實驗這麼耗時耗力的事兒，可不可以不做呢？如果評審意見符合以下三種情況，那麼最靠譜的做法就是儘快完成實驗補充。
1.如果補實驗這個問題是幾個審稿人提出的共同問題，那麼各種童鞋應該嚴肅對待，且必須回答和進行實驗補充。
2.期刊會將你的回覆返回給原審稿人時，而非新審稿人時，此時也需進行實驗補充；
3.期刊若只給一次返修的機會，那也必須補實驗了。
除此之外，如果你真的不想補實驗，就需要儘量跟審稿人據理力爭一番，當然此時答覆審稿人時仍需要保持一種尊重的態度。
1）以往的文獻若已經能回答審稿人的問題、支持你不補實驗時，可在和審稿人爭論時旁徵博引（其他文獻），並在報告中補充過程和實驗細節，同時可補充一些相似實驗來增加一些容易實現的實驗結果來增強審稿人信心。
舉個栗子：Comment #6: 文章需要補充正常皮膚作對照
回覆：Indeed, it will be more convincing if we get a comparativeassessment on normal keratinocytes. However, the normal skin cell line TE353.SK that we recently obtained could not be cultured stably under thecondition of our lab at this time. We are also collecting human tissue samplesfrom patients with primary melanoma and benign nevi. Based on our data, XXXexpression will be analyzed in clinical samples by immunohistochemistry, andthe correlation between XXX expression and the prognosis of melanoma will bedetermined in our subsequent investigations. Therefore, the referee’s concernis of importance for our further study, and we will show the results in ournext paper for XXX.
2）補充動物實驗，是審稿人最愛發的一招。此時只能見招拆招，如果能補上自然是給了漂亮的回馬槍；但是若是實驗條件有限或者時間來不及，根本無法滿足審稿人的要求時，也要儘量在回覆裡委婉的說明客觀原因，表明自己確實努力過了，但是事與願違啊！
此外，寫作者還需要說明將來一旦條件可行了，則必然完整進行這個實驗；並將審稿人的這一問題當作本文的一個不足，在Discussion裡討論一下，也是可以的。
舉個栗子，Comment #7: 文章需要補充動物實驗
回覆：We are appreciative of the reviewer’s suggestion. Indeed, it will be more profound if we get therelevant results in vivo. We have purchased 10 thymus-null BALB/c nude mice(female, age 4-6 weeks) from the Animal Center of Chinese Academy of MedicalSciences. XXX tumor were established in nude mice according to a previousreport (Gao et al., 2009) （我們去買了若干只小鼠，按照文獻的方法操作）. Briefly, …（實驗操作方法）.Unfortunately, the tumours could not grow after inoculation for three weeks（滿懷欣喜的接種到動物身上，然而實驗這東西，哪能一遍就成呢，我們做了一遍，結果沒能成瘤，實在是悲劇了）. The limitation of technical condition may be the main reason.Also, we doubt that the cell viability could be insufficient to initiateosteosarcoma tumorigenesis in vivo due to the long-distance transport.Therefore, we seek for the editor’s tolerance andunderstanding. Many thanks for your kind help!
3）投稿的文章已經能夠說明某個問題了，最後表明要求補的實驗正在做，在以後的工作中會繼續研究。
舉個栗子，Comment #8: 文章需要補充某個具體實驗
回覆：“We understand that…（要補充的實驗）maybetter reveal the…However, in the presentstudy, we mainly focused on…（本文研究重點）, andwe think that…（已有的實驗）may not beoptimal, but should be sufficient to draw a conclusion that…（研究得出的結論）”或者“The…（某個實驗）underinvestigation in our laboratory. Unfortunately, results are unavailable at thispoint.”
4）有時候，補充實驗結果真的沒那麼重要，也可以在最後的Discussion裡淡化這方面的討論，並回答審稿人“We agree with the reviewer, and have toned down our conclusions.”
5）最後，儘量把校正的部分一一列在回覆報告裡相應的地方，而不要讓審稿人到文章中去找你修改的地方，且儘量使得文章格式符合該期刊格式。
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