小夥伴們，SCI文章寫作到了最後，是不是最頭疼如何討論？我的實驗結果槓槓的、我的方法有所欠缺、或者別人的相關研究有了新的進展…這些都怎麼愉快的表達呢？莫慌，今天給大家帶來一些討論方面的寫作例句，希望可以幫助到毫無方向的一些新手！
M方法 A 病名 X藥名 C細胞
－－－討論－－－
效果好
The M produced the best results.
These results are far better than those of adult DMs cultured with TIC medium, and are comparable to neonatal DMs cultured with TIC medium.
UM can be successfully cultured from eyes with A.
The best results were obtained by M; using this method, the IPE was separated by microdissection after treatment with an enzyme solution, which decreased the trauma produced by prolonged exposure to the enzyme and the mechanical injuries caused by the direct dissection method.
The M, which yields the best results for isolating IPE from autopsy eyes, yielded better results for the surgical specimens.
藥物效果好
Sb reported that X at a concentration of 100 ug/ml eliminated fibroblasts without affecting DM.
細胞好（做試驗可靠）
UMs could be cultured in FIC medium with a doubling time of 2.3 days, divided for 40-50 times and maintained over a long period (up to 7 months).
試驗結果的可靠性
These experiments were repeated several times, and were carried out under double blind conditions in Experiments 1 and 2. All results were in good agreement, indicating the results are valid and repeatable.
The results were reproducible and the effects were consistent for all adrenergic agonists tested.
Furthermore, our results are consistent with the results of the binding assays showing that---.
毒性
X is more toxic to rapidly dividing cells.
表達方法：以往的不足
Early attempts to establish UM in culture were frustrated largely by the difficulty of isolating melanocytes from uveal tissues, their slow growth in vitro, and the proliferative advantage of other cell types in the culture.
Earlier researchers used explant methods to isolate UM, but their outgrowth was slow and few UM grew.
We tried this method, but only UM from the outer lamina could be isolated, isolation was incomplete, and cell yields were low.
注意事項
To establish pure cultures of UM, it was necessary to address three problem areas: methods of isolation, elimination of contaminant cells, and growth stimulation.
新進展
In recent years, some authors used Ms to isolate UM from the uvea.
We have developed a method to separate pigment epithelium and sclera from uvea first, facilitating isolation of UM from the uvea completely with little or no contamination by pigment epithelium and scleral fibroblasts.
The involvement of the a-adrenoceptor in melanogenesis may be regarded as unexpected, since it is typically regarded as being associated with ---. This provides yet further evidence that the a-adrenoceptor is well represented in the human eye.
優點
This method has several advantages.
The M led to higher cell yields and less cell damage than the M2.
The advantages of the enzyme microdissectionenzyme isolation method include the following: (1) Isolating the IPE as a complete sheet, avoiding the loss of cells and resulting in a 63.8% increase in cell yields. (2) Separating the IPE with fine forceps, reducing trauma. (3) Decreasing the time needed for exposing the cells to the enzyme solution (10 minutes before and 30 minutes after separation) vs the M2(90 minutes), reducing cell damage from prolonged exposure to enzymatic digestion. These factors resulted in higher cell yields, better viability, and better growth ability of the IPE isolated by the M2.
It is easy to measure in living cells; the results are reliable and repeatable, and has been widely used as a parameter for evaluating the effects of various factors in differentiation of Cs in vitro.
解釋說明
Several explanations exist for the incomplete cell yields, the low viability, and the low growth rate. First, the excised tissue is small, containing less than 1% of the IPE of the whole iris. It is more difficult to induce cells to proliferate when the cell density is low compared with when a large population of cells is available. Second, the loss of cells during the isolation process further decreases the success rate. The tiny specimens make it more difficult to manipulate and easier to injure the IPE cells. Third, surgical trauma also decreases cell viability. Therefore, a refined technique was required to obtain better results.
One possible explanation for this discrepancy may be that --- and that --- in the short duration of the present study.
意義
Measurement of melanin content is more direct and meaningful/（important）than that of tryosinase activity.
This method provides a valuable source of large numbers of Cs, which can be used to study their biological behavior, to compare with the UMs; and to compare them to their malignant counterparts in the exploration of the pathogenesis of A.
The demonstrated synergistic effect of antioxidants and trophic factors may have important clinical implications. Future studies of different combined treatments might provide new avenues to combat RGC death in IOP-elevated eyes.
－－－結論－－－
表達方法：新方法及其優點
In conclusion/(summary), we have developed a method for isolating and cultivating UM that yields consistently satisfactory results.
This method provides a valuable source of large numbers of adult-derived UM, which can be used to study their function and structure in vitro.
Such cultures may be useful in investigations of the role of UM in the pathogenesis of various eye diseases, including A1, A2, and others.
The study of cultured IPE from the surgically excised iris of patients with various diseases may improve our understanding of their pathogenesis.
Comparing the metabolic processes between normal IPE and cultured IPE taken from patients with A will be the basis for further understanding the pathogenesis of A.
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